IMMUNOLOGY2024™

May 3–7, 2024 | Chicago, Illinois

Postdoctoral Fellow

Long Island University College of Veterinary Medicine
Brookville, New York, USA

Postdoctoral Fellow Position in CD8 Regulatory T Cells in Multiple Sclerosis.

Dr. Xiaolei Tang’s laboratory in the Department of Biological Sciences at Long Island University College of Veterinary Medicine is seeking a highly motivated, qualified individual to investigate the role of CD8 regulatory T cells in multiple sclerosis.

The incumbent of this postdoctoral fellow will participate in an industry-sponsored project aiming to develop a novel therapy to stop multiple sclerosis progress by enhancing the function of CD8 regulatory T cells. The fellow will gain experience working in an academic research laboratory while bridging basic research and clinical drug development. The research laboratory is fully equipped with state-of-the-art instruments essential for daily research activities, e.g., Cell Culture Biosafety Cabinet, CO2 Incubator, Flow Cytometer, ImmunoSpot Analyzer, Freeze Lyophilizer, Nanoparticle Analyzer, Confocal Microscope, Gas Chromatography among others. You may access the research activities in Dr. Tang’s laboratory by following this link.

The fellow will also participate in research activities at a private startup biotech company.

LIU-CVM (https://liu.edu/Vetmed) is located on LIU Post campus and has an interdisciplinary research program with highly accomplished scientists and board-certified veterinary pathologists. The research program comprises basic, translational, and clinical research with combined cellular, molecular, genetic, and clinical sciences expertise. Research focuses include infectious diseases, autoimmune disorders, cancers, and tissue regeneration. The research program aims to improve the understanding of disease pathogeneses and utilize the newly gained knowledge on disease pathogeneses to develop novel therapeutics. Newly renovated research laboratories within the LIU-CVM are equipped with state-of-the-art instruments for the above-mentioned research activities.

Specific duties include (but are not limited to):

Cell Culture:

  • Set up cell culture for primary cells and cell lines.
  • Transduce cells with lentiviruses.
  • Perform assays to evaluate cell functions such as proliferation, cytokine secretion, apoptosis, and differentiation.

Flow Cytometer:

  • Performing various analyses using flow cytometers such as cell subset and cycle analyses.

Molecular Biology

  • Isolate DNA and RNA.
  • Perform agarose gel electrophoresis, Cloning, PCR, real-time RT-qPCR, ChIP, ChIP-Seq.
  • Knockdown genes.
  • Overexpress genes.

Biochemistry

Isolate and quantify proteins.Perform SDS-PAGE, western blotting, immunoprecipitation, in vitro and in vivo biochemical assays.

Immunohistochemistry

  • Prepare paraffin-embedded and cryosections.
  • Perform histology, H&E staining, and immunohistochemistry staining.

Microscopy

  • Analyze images using bright field, fluorescence, and confocal microscopes.

Spectroscopy

  • Use UV-Vis spectroscopy and spectrophotometry to quantify DNA, RNA, and protein.

Bioinformatics

  • Design primers.
  • Perform sequence alignment.
  • Bulk RNA-seq and scRNA-seq.
  • Analyze gene sequencing data using machine-learning techniques.

Small Animal Handling

  • Breed and maintain mouse colonies.
  • Genotype for knockout and transgenic mouse models.
  • Perform animal procedures such as isolation of intestinal crypts, intravenous/intraperitoneal/subcutaneous injections, necropsy and tissue collection, and immunohistochemistry of tissue sections.
  • Perform luciferase imaging of live animals.

Mentoring

  • Mentor undergraduate, Doctor of Veterinary Medicine, Master, and PhD students.

Publishing

  • Gather and interpret experimental data, preparing reports and manuscripts.
  • Perform other duties as assigned.

The Successful Applicant:

  • Will have at least one year of experience directly related to the above-assigned duties.
  • Will demonstrate excellent organizational skills.
    • Willing to work on multiple projects.
    • Work effectively with other laboratory members.
  • Willing to work effectively with other faculty, staff, and administrators as necessary.
  • Will demonstrate the ability to independently carry out significant projects critical to the university and community.
  • Will have a solid and consistent publication record in tissue repair and/or immunology.

Education Requirements

  • Minimal qualifications include a Ph.D. in natural science or a health-related field from a college or university of recognized standing.

Application Instructions

  • Cover letter, resume, transcripts if recently graduated, personal statement, research statement, and future research plan.
  • Contact: xiaolei.tang@liu.edu.

LIU is an equal-opportunity employer. LIU is committed to extending equal employment opportunities to all qualified candidates who can contribute to the diversity and excellence of our academic community. LIU encourages applications from all qualified individuals without regard to race, color, religion, genetic information, sexual orientation, gender and/or gender identity or expression, marital or parental status, national origin, ethnicity, citizenship status, veteran or military status, age, disability or any other basis protected by applicable local, state or federal laws. Hiring is contingent on eligibility to work in the United States.

Posting ID: 417