Research Manager—Dr. Klaus Ley Lab
Immunology Center of Georgia at Augusta University
Augusta, Georgia, USA
Immunology Center of Georgia
The Immunology Center of Georgia (IMMCG) represents a new initiative by the Medical College of Georgia at Augusta University to leverage the ability of the immune system to combat disease. The IMMCG will accomplish this by assembling teams of biomedical researchers with strong records of scientific innovation and technological development. The IMMCG will be co-directed by world-class immunologists Drs. Catherine ‘Lynn’ Hedrick and Klaus Ley, both of whom have made major discoveries linking immune cells to specific mechanisms of disease. The goal of the IMMCG is to produce novel and effective treatments for conditions that heavily impact the communities of Georgia and the United States. The new center is aggressively recruiting faculty and staff to lead projects focused on cardiovascular disease, obesity and metabolic disorders, autoimmune and infectious disease, neurodegenerative disease and cancer.
Medical College of Georgia
The Medical College of Georgia is one of the nation’s largest medical schools by class size, with 260 students per class. The educational experience is anchored by the main campus in Augusta, regional clinical campuses for third- and fourth-year students across the state and a second four-year campus in Athens in partnership with the University of Georgia. MCG’s expanding partnerships with physicians and hospitals across Georgia currently provides about 350 sites where students can experience the full spectrum of medicine, from complex care hospitals to small-town solo practices. MCG and its teaching hospitals also provide postgraduate education to more than 500 residents and fellows in 50 different Accreditation Council for Graduate Medical Education-approved programs. Our researchers and clinicians focus on what most impacts the health of Georgia’s and America’s children and adults, including cardiovascular biology and disease, cancer, neurosciences and behavioral sciences, public and preventive health, regenerative and reparative medicine, personalized medicine and genomics. Our physician faculty also share their expertise with physicians and patients at about 100 clinics and hospitals statewide.
Job Summary
Administrative accountability for leading, managing, developing, supervising, and evaluating all research personnel and supportive research staff. Manages and collaborates relationships with research staff to ensure professional and educational excellence in provision of quality research. Responsible for supervising, training and evaluation of supportive research staff. Collaborates with Grants/Fiscal Managers and designees to develop and maintain a balanced budget.
Responsibilities
Responsibilities include, but are not limited to:
Managerial Duties
- Oversee postdocs and technicians involved in single cell RNA sequencing
- Interface with the sequencing facility (currently Eiko Kitamura)
- employee development
- interviewing candidates
- scheduling experiments
- training staff
- attending technical meetings with 10x Genomics, BD Rhapsody and other relevant companies
- organizing the ordering of all supplies for scRNA-Seq, including
- Library prep reagents
- Gel beads
- Antibodies
- TCR and BCR kits
- manage all stocks to avoid expiration dates and maintaining the lab,
- Maintain inventory of all PBMC samples in LN2 dewars
- Maintain inventory of CITE-Seq antibodies
- Maintain inventory of reagents
- Perform cost analyses of scRNA-Seq experiments
- Calculate sequencing needs
- Optimize use of Illumina flow cells
- Maintain good communication with all lab members and Klaus
Lab Duties
- Isolate PBMCs from human blood
- Thaw PBMCs using established SOP
- Organize cell sorting using the flow cytometry core
- Sort cells as needed
- Stain with hashtags, antibodies and wash appropriately
- Plan all scRNA-Seq experiments
- Plan all bulk RNA-Seq experiments
- Measure cell number and viability
- Establish all pre-sequencing quality controls
- Prepare libraries for mRNA, antibodies, TCR, BCR as needed
- Run Tapestation
- Receive FASTQ files from sequencing
- Run Seven Bridges or Cell Ranger as needed
Other Duties
- All other related duties/task as assigned
Posting ID: 427