Exhibitor Workshops

Speaker
Egon Ranghini, Ph.D., Senior Science & Technology Advisor, 10x Genomics

See how 10x Genomics is helping researchers uncover molecular insights from fresh or fixed samples, including FFPE tissues. Explore cellular heterogeneity with Chromium Single Cell solutions that let you dissect cell-type differences, investigate the innate and adaptive immune systems, identify rare cell types, detect novel biomarkers, and map the epigenetic landscape cell by cell.

10x Genomics

Speakers
Deena Soni, Ph.D., Associate Director Product Marketing, Sony Biotechnology Inc.
Koji Futamura, Ph.D., Senior Manager, Sony Biotechnology Inc.

The new FP7000 Spectral Cell Sorter features state of-the-art spectral optics, electronics, and fluidics that deliver high resolution, sensitivity, and performance for sorting heterogenous populations. In this talk, we highlight the design and key capabilities of the FP7000 Spectral Cell Sorter including intuitive software tools that streamline sorting. Using high parameter immunotyping panels, we will discuss high speed sorting results and how panels optimized on the ID7000 Spectral Cell Analyzer can be setup for sorting on the FP7000 Spectral Cell Analyzer.

Sony Biotechnology Inc.

Speaker
Robert (Bob) Balderas, VP Biological Sciences, BD Biosciences

Understanding biology with flow cytometry relies on antibody conjugates and distinguishable fluorescent agents combined into panels built to reveal expression levels of a varied set of receptors on suspended cells. Advancements in flow cytometry increased the number of possibilities of antibody conjugates to be used simultaneously in a panel. In this talk, as BD celebrates 50 years of innovation in flow cytometry, we'll share our learnings through a candid discussion on how decisions around panel design and experimental conditions impact biological resolution.

BD Biosciences

Speaker
Mehdi Damaghi, Ph.D., Principal Investigator, Stony Brook Medicine, Stony Brook University

A conducive microenvironment is crucial to cancer initiation and formation of a tumor, with a growing emphasis on understanding the evolutionary trajectories of precancerous cells in their variable ecosystems. Here we provide a detailed eco-evolutionary atlas for DCIS progression and upstaging. We developed multiplex IF panels for staining and analysis on the MACSima™ Platform to characterize the habitats for 3D spheroids, organoids, and patients’ samples. Spatial proteomic data was integrated with MALDI mass spectrometry metabolomics and lipidomics to produce a detailed breast cancer landscape profile.

Miltenyi Biotec

Speakers
Brandon Trent, Ph.D., Senior Applications Scientist, Thermo Fisher Scientific
Dan Quigley, MSME, Lead Systems Design Engineer, Thermo Fisher Scientific,

As the field of flow cytometry expands our knowledge of the immune system, increased panel complexity has put a greater demand on instrumentation. Spectral unmixing has advanced panel size and diversity, enabling the study of novel cell populations. To help meet these needs, we have developed the Invitrogen™ Attune™ Xenith™ Flow Cytometer to bring clog-resistant acoustic focusing technology to high parameter flow cytometry. This system enables the use of spectral unmixing and high parameter compensation at speeds up to 500 µL/minute with lnvitrogen™ CytKick™ Autosampler compatibility.

Thermo Fisher Scientific

Speaker
Zayda Piedra, Ph.D., Scientist II, Scientific Applications Group, BioLegend

To characterize cell function and understand pathological conditions, technologies evolve to provide better ways to interrogate biological systems. One such emerging tool is spectral flow cytometry. Capable of multiplexing > 40 parameters, the availability numerous reagents makes it challenging to build optimal solutions. Here, we discuss strategies utilied by BioLegend to build complex panels, including backbone combinations with open channels for easy expansion. Thus, BioLegend’s  unique reagents and experience can help your research with pre-optimized panels or a customized design.

BioLegend

Speaker
Morgan R. Little, MBS, Technical Sales Consultant, Miltenyi Biotec

While the CAR T cell therapy product pipeline has grown exponentially over the last decade, researchers continue to confront obstacles when moving from developmental stages to pre-clinical and clinical studies. One of these obstacles is the learning curve involved with increasingly robust and standardized assays. During this workshop, we will delve into the lessons gleaned from developing a CAR T cell therapy product, the workflow for successful translation from a small- to large-scale therapeutic, and how Miltenyi Biotec provides researchers with innovative solutions.

Miltenyi Biotec

Speakers
Holden T. Maecker, Ph.D., Professor (Research), Microbiology & Immunology, Stanford University
Max Mahoney, Regional Sales Director-East, Sales, Alamar Biosciences

The NIH IMPACC study followed over 1,000 hospitalized COVID-19 patients from admission through convalescence. In this study, we used a 250-plex inflammatory cytokine panel (NULISAseq) to augment the previously completed serum cytokine profiling using the Olink Target 96 inflammatory panel. Using multivariate modeling, we determined predictors of severity from baseline and longitudinal measurements during the acute hospitalization period. We could rapidly profile thousands of samples with high sensitivity and precision using the automated ARGO HT instrument (Alamar Biosciences).

Alamar Biosciences

Speaker
Eleanor Kincaid, Ph.D., Field Applications Manager Eastern US, Cytek Biosciences

As the identification of new immune cell subsets continues, the demand for high-parameter flow cytometry is steadily increasing due to the growing number of markers that need to be measured per cell. This tutorial will demonstrate how FSP™ technology enables a deeper and more accurate immune system characterization. We will discuss the methodology utilized to develop a high-quality human 45-color panel that allows for comprehensive characterization of the major cell lineages present in circulation, including CD4 T cells, CD8 T cells, regulatory T cells, γδ T cells, NKT-like cells, B cells, NK cells, monocytes, and dendritic cells, that also provides outstanding granularity of cell activation, exhaustion, memory, and differentiation in CD4+ T cells, CD8+ T cells, and NKT-like cells. We will present data highlighting the ability to use this panel across sites, achieving high concordance in resolution and agreement in results across 5 Laser Cytek Aurora™ flow cytometers and the Cytek Auora™ CS system. In addition, we will discuss how this panel can be used for cell sorting and enables functional characterization of specific rare cell subsets. In summary, with FSP technology and our assay optimization workflow, we can develop high resolution multiparametric assays that can be immediately applied for sorting rare populations. This approach offers the opportunity to fully characterize immunological profiles present in peripheral blood in the context of infectious diseases, autoimmunity, neurodegeneration, immunotherapy, and biomarker discovery.

Cytek Biosciences

Speakers
Amanda Durkin, Ph.D., Product Manager, Immunology, STEMCELL Technologies Inc.
Manreet Chehal, Ph.D., Scientist, STEMCELL Technologies Inc.

Due to their central role in the adaptive immune response to cancer and foreign pathogens, T cells are a focal point of fundamental and translational research. To remain ahead in these constantly changing fields, it is crucial to implement more efficient and scalable processes. Join this workshop to learn about next-generation T cell isolation and expansion tools from STEMCELL Technologies that can streamline your research. We'll feature the Easy 250 EasySep™ magnet for efficient large-volume, column-free cell isolation, and new cGMP ImmunoCult™ products to activate and expand T cells.

STEMCELL Technologies Inc.

Speaker
Tyler Hulett, Ph.D., Chief Scientific Officer, CDI Labs

Each individual’s serome is incredibly unique, complex, and yet accessible via proteome microarrays (HuProt), phage-display immunoprecipitation sequencing (PhIP-Seq, HuScan, VirScan), and other new synthetic biology platforms. Seromics experiments have revealed a viral-induced autoimmune cause of multiple sclerosis, pre-treatment autoantibodies associated with checkpoint blockade immunotherapy outcomes, and the demonstration that autoantibodies can mimic inborn errors of immunity – genetic-knockout-like phenotypes only discoverable via antibody profiling.

CDI Labs

Speakers
Leilani Marty Santos, Ph.D., Spatial Science & Technology Advisor, 10x Genomics
Lynn Van Olst, Ph.D., Postdoctoral Research Fellow, David Gate Laboratory, Northwestern University

Spatially resolved biology, including whole transcriptomic and targeted in situ methods, allows scientists to build a more complete view of cellular function in a morphological context, representing a paradigm shift in the study of biological systems. Visium from 10x Genomics is a NGS-based spatial discovery platform that allows whole transcriptome profiling of tissues. The Xenium In Situ platform is an imaging-based solution that provides precise localization of thousands of RNA targets with subcellular resolution, offering true single cell spatial analysis.

10x Genomics

Speaker
Winfried Wiegraebe, Product Manager, Super-Resolution Microscopy, Bruker

Super-resolution microscopy techniques such as PALM, dSTORM, and DNA-PAINT, allow us to see structures smaller than the diffraction limit at a resolution crucial in immunology. The Bruker Vutara VXL microscope can help uncover these mysteries at the coverslip and within tissue. It uses single-molecule localization microscopy (SMLM) principles, with a patented bi-plane technology for super-resolution z-stacks. It includes integrated fluidics for multiplexing and two cameras for multi-color imaging. The Vutara SRX software guides users through experiments and provides tools for analysis.

Bruker

Speakers
David Hamm, Parse Biosci.
Efi Papalexi, Ph.D, Staff Scientist, Parse Biosci.
Ajay Sapre, Parse Biosci.

Unlock the intricate complexity of the immune system when explored through the lens of today’s leading single cell RNA-Sequencing (scRNA-Seq) techniques. In this session, we explore the unprecedented resolution of immune cell diversity when explored at a previously unattainable scale. These new tools are set to empower the next wave of insights with applications in the acceleration of drug discovery, drug development, and modern therapeutics.

Parse Biosciences

Speaker
John Quinn, Ph.D., Director, Science and Product Development, Technical Consulting, BD Biosciences

In this talk we will discuss the full workflow to take the most direct deep dive into your data set using FlowJo™ Software. We will use the recent monumental 50-color spectral data set from Konecny et. al. at the Fred Hutchinson Cancer Center as an example to delve into scaling, compensation options, and automated clean-up, as well as show the application of a high-dimensional workflow centered around the T-rex algorithm for identifying populations of interest and automated population naming. Join us to walk through the cutting edge of cytometry.

BD Biosciences

Speakers
J. Spencer Schwarz, M.S., Manager, Discovery Applications, Canopy Biosciences
Mara De Martino, Ph.D., Postdoctoral Associate, Canopy Biosciences

Spatial Biology is expanding frontiers in immunology. Here we introduce CellScape™ Precise Spatial Multiplexing as a powerful tool to support complex interrogation of tissue samples. Dr. Mara De Martino of Weill Cornell Medicine will discuss ongoing work to improve the outcomes of radiation therapy in patients with glioblastoma. This work incorporates data generated with CellScape to understand the population dynamics of tumor infiltrates as part of a multi-modal approach that links tumor metabolism modulation to immune responses, generating actionable insights toward improving therapy efficacy.

Canopy Biosciences — A Bruker Company

Speaker
Stefan Jellbauer, Ph.D., Senior Manager, Product Management Immunoassays, Thermo Fisher Scientific

The  COVID-19 pandemic, caused by SARS-CoV-2, put the terms “immune response” and “immunology” on everyone’s radar. There is an urgent need to understand the immune response and relevant immunological mechanisms underlying the progression and long-term effects of many diseases, including COVID-19. This workshop introduces advancements in multiplex analysis of RNA and protein using the Luminex® xMAP® technology. Leveraging Invitrogen™ ProcartaPlex™ and QuantiGene™ Plex assays overcomes limitations by allowing simultaneous screening of up to 80 biomarkers. These high-plex assays help save precious sample, time, and cost.

Thermo Fisher Scientific

Speakers
Anthea Nice, Ph.D., Product Manager, Immunology, STEMCELL Technologies Inc.
Trevor Rogers, Scientist, STEMCELL Technologies Inc.

Adopting an efficient T cell workflow is essential to advancing mouse immunology research, from fundamental research to generation of complex disease models. Join this workshop to learn about STEMCELL Technologies’ new streamlined solutions for mouse T cell isolation and expansion. We will feature our new EasySep™ Release Mouse CD4+CD304+ Regulatory T cell isolation kit, which enables fast, column-free isolation of regulatory T cells without targeting CD25. We will also highlight our new soluble ImmunoCult™ Mouse T Cell Activators, which effectively activate and expand mouse T cell subsets.

STEMCELL Technologies Inc.

Speaker
Sharon Sanderson, Ph.D., Flow Cytometry Application Scientist and Product Manager, Bio-Rad Laboratories, Inc

Advances in technology and fluorescent dye diversity means there are more complexities to navigate during experimental design. Following a 7-step process, we have generated human and mouse no/low compensation immunophenotyping panels. These panels generate data of a high quality that simplifies analysis; they work as a backbone framework to build higher plex panels and save on reagents and time. We will show you how you can build your own no/low compensation panels for conventional instruments, that will also work for spectral systems, enabling you to generate reliable and reproducible results.

Bio-Rad Laboratories, Inc

Use of large animal cohorts can be required to perform standard immune repertoire and transcriptome profiling, especially for time-course and dose-response studies in murine models. This workshop will describe methods using small volume blood samples for profiling T- and B-cell receptor repertoires and transcriptomes in mice at different stages of tuberculosis progression, an approach that provides biologic insights while reducing animal usage.

Cellecta

Speaker
Josh Koenig, Ph.D., Assistant Professor, McMaster University, Immunology Research Centre

How IgE secretion drives food allergy remains poorly understood. Using an early-stage model of food allergy, we found that a single gavage of allergen with cholera toxin adjuvant failed to sensitize mice, but allergen re-exposure, even one year later, caused sensitization and anaphylaxis. Initial exposure induced CD44^hi lineage-ambiguous CD4+ T cells but failed to activate B cells. Allergen re-exposure drove T-follicular helper cell differentiation and de novo IgE production. Our data implicate memory CD4+ T cells in initiating IgE production and holding IgE memory.

BioLegend

Speaker
Avik Mujherjee, Ph.D., Senior Technical Applications Scientist, Akoya Bio

Discover groundbreaking insights into the immune microenvironment and the transformative impact of spatial biology on immunology and clinical practice. Join Dr. Avik Mukherjee and explore Akoya’s innovative Spatial Biology 2.0 solutions, which revolutionizes precision medicine on a scale never seen before. Dr. Mukherjee will delve into technologies such as PhenoCycler^®-Fusion 2.0, PhenoImager^® HT 2.0, and PhenoCode™ Panels, and discuss their deployment from high-plex discoveries to actionable signatures across the research spectrum.

Akoya Bio

Speaker
Oliver J. Kreuzer, Ph.D., CEO, peptides&elephants GmbH

The Covid-19 pandemic led to unprecedented development  of RNA vaccines, which provide protection from severe  diseasecourses. Peptide pools are essential for testing whether individual proteins are suitable antigen candidates and are important tools for evaluating adoptive T cell immunity.  In this workshop, Dr. Kreuzer reviews the exciting work of peptides&elephants GmbH in the pandemic – the mapping of the spike glycoprotein, the different variants via the rapid production of peptides using ultrasound peptide synthesis, and the assembly of complete peptide pools./

peptides&elephants GmbH