Saturday, May 4, 2024     2:15 PM – 3:00 PM ET     Exhibitor Workshop Room 2

High Resolution In-Depth Characterization of the Human Memory T Cell Compartment Using Full Spectrum Profiling™ (FSP™) Technology

Speaker
Eleanor Kincaid, Ph.D., Field Applications Manager Eastern US, Cytek Biosciences

As the identification of new immune cell subsets continues, the demand for high-parameter flow cytometry is steadily increasing due to the growing number of markers that need to be measured per cell. This tutorial will demonstrate how FSP™ technology enables a deeper and more accurate immune system characterization. We will discuss the methodology utilized to develop a high-quality human 45-color panel that allows for comprehensive characterization of the major cell lineages present in circulation, including CD4 T cells, CD8 T cells, regulatory T cells, γδ T cells, NKT-like cells, B cells, NK cells, monocytes, and dendritic cells, that also provides outstanding granularity of cell activation, exhaustion, memory, and differentiation in CD4+ T cells, CD8+ T cells, and NKT-like cells. We will present data highlighting the ability to use this panel across sites, achieving high concordance in resolution and agreement in results across 5 Laser Cytek Aurora™ flow cytometers and the Cytek Auora™ CS system. In addition, we will discuss how this panel can be used for cell sorting and enables functional characterization of specific rare cell subsets. In summary, with FSP technology and our assay optimization workflow, we can develop high resolution multiparametric assays that can be immediately applied for sorting rare populations. This approach offers the opportunity to fully characterize immunological profiles present in peripheral blood in the context of infectious diseases, autoimmunity, neurodegeneration, immunotherapy, and biomarker discovery.

Cytek Biosciences